Protein Microarray Immobilization via Epoxide Ring?Opening by Thiol, Amine, and Azide

Immobilization of functional molecules in ordered microarrays has vast importance for many applications sensing and diagnostics. Here, the immobilization small molecule compounds protein microchannel cantilever spotting (?CS) on epoxy-terminated glass surfaces is studied via ring-opening epoxides by thiol, amine, azide, with purpose creating microscale patterns applications. For this, carrying epoxy groups from silanization are functionalized ?CS different fluorescent nonfluorescent dyes containing or azide groups. Experiments fluorophores reveal that all routes result successful a short time. Furthermore, these experiments, optimal reaction conditions including time, temperature, catalyst type, amount determined. The feasibility examined means binding experiments. Comparing fluorescence intensity values biotin spot arrays immobilized surfaces, after incubating fluorescent-labeled streptavidin, highest surface density amine route comparison to thiol routes. obtained results this work can inform design fabricating biosensors as well other biomedical diagnostic